Food allergy and anaphylaxis
Carmine carries proteins from the insect that can make the immune system react. Case reports since the 1990s describe anaphylaxis after eating carmine-coloured yoghurt, ice lollies and alcoholic drinks. In one case, as little as 1mg set off a reaction. The trigger is the insect protein, not the red pigment itself.
Anaphylaxis after eating carmine-coloured yoghurt was shown to be an immune (IgE) reaction by skin prick and blood tests. One person reacted to 1mg of carmine.
1995ObservationalBeaudouin E et al., Annals of Allergy, Asthma and Immunology, volume 74, 427-430
Anaphylaxis from an ice lolly was confirmed as an allergy to carmine by skin prick testing, and insect proteins, not carminic acid, were identified as the trigger.
1997ObservationalBaldwin JL et al., Annals of Allergy, Asthma and Immunology, volume 79, 415-419
Among 110 people with hives or suspected additive reactions, 8% (9 people) had carmine allergy confirmed by eating it under supervision. All had a long-term type of hives set off by things like pressure or cold.
2022ObservationalSadowska B et al., Postepy Dermatol Alergol, volume 39, 94-100
Asthma in workers who breathe it in
Workers who breathe in carmine dust in dye, food or cosmetics factories often become sensitised, and some develop work-related asthma. In one factory, over 40% of current workers were sensitised and 8% had asthma. Counting workers who had already left because of illness, the asthma rate was higher. The same insect protein drives both the breathing allergy and the food allergy.
In a natural dye factory, 41.7% of current workers had positive skin tests to carmine and 8.3% had work-related asthma. Including three former workers who had left, sensitisation rose to 48.1% and asthma to 18.5%.
2003ObservationalTabar-Purroy AI et al., Journal of Allergy and Clinical Immunology, volume 111, 415-419
Carmine is a confirmed skin and breathing sensitiser, and European reviewers concluded that breathing it in during handling is very likely.
2022Regulator reviewEFSA FEEDAP Panel, EFSA Journal 20(10):7609
Dust mite and shellfish allergy
Cochineal insects are relatives of dust mites and shellfish, so some researchers thought carmine allergy depended on being allergic to those. Studies found dust mite allergy is common in people allergic to carmine, but carmine reactions can happen without it. Newer tests using purified carminic acid avoid the confusion.
Of 3,164 people skin-tested, 94 (3%) reacted to carmine. 74% of those also reacted to dust mites, but reactions after eating did not depend on mite allergy in 42% of cases, and about a quarter had carmine allergy with no mite allergy at all.
2009ObservationalLiippo J and Lammintausta K, International Archives of Allergy and Immunology, volume 150, 179-183
Standard tests for cochineal allergy are confused by cross-reactions with shellfish and dust mites. Tests using carminic acid give a much more specific diagnosis.
2022ObservationalGanseman S et al., Clinical and Experimental Allergy, volume 52, 1225-1229
The daily limit does not protect allergic people
Both the international committee and European reviewers set a daily limit of 5mg of carmine per kg of body weight. That limit is about toxicity, not allergy. Someone who is allergic can react to far less.
A long-term rat study found no harm at 500mg of carmine per kg of body weight a day.
2015Regulator reviewEFSA ANS Panel, EFSA Journal 13(11):4288
The international committee confirmed a daily limit of 5mg per kg of body weight for carmines, but set none for cochineal extract because the data were not enough at the time.
2001Regulator reviewJECFA, WHO Food Additives Series 46
Linked to more type 2 diabetes in a large French study
The NutriNet-Santé study followed 108,723 French adults for about 8 years and tracked the colours in the packaged food they ate. People who ate the most cochineal and carmines (E120) were 27% more likely to develop type 2 diabetes than those who ate little or none. This is one observational study.
Among 108,723 French adults followed from 2009 to 2023, higher intake of cochineal and carmines as a food colour was linked to a 27% higher risk of type 2 diabetes (hazard ratio 1.27).
2026Observational study in peopleNutriNet-Santé cohort, Shah et al., Food Coloring Additives and Incidence of Type 2 Diabetes, Diabetes Care