Anthraquinones in the raw plant
The cassia plant naturally produces anthraquinones, a class of compounds that at high doses act as stimulant laxatives and have shown genotoxic (DNA-damaging) effects in laboratory tests. This is why regulators required a purification step before authorising the gum for food use. The specification of less than 0.5mg anthraquinones per kg of finished gum was set as the threshold below which the food additive is considered not to present a safety concern at permitted use levels.
EFSA's Panel on Food Additives concluded that cassia gum meeting the specification of anthraquinones below 0.5mg per kg does not raise a safety concern at the proposed food uses and levels.
2006Regulatory reviewEFSA Panel on Food Additives and Nutrient Sources (ANS), Scientific Opinion on cassia gum
EFSA's feed additive panels reiterated that only semi-refined cassia gum meeting the food-additive specification (anthraquinones below 0.5mg per kg) can be considered acceptable; higher anthraquinone content is not acceptable.
2017Regulatory reviewEFSA FEEDAP Panel, Safety of cassia gum as a feed additive for dogs and cats (Intercolloid dossier)
Genotoxicity of anthraquinones
Several anthraquinones found in cassia, including emodin, have shown positive results in bacterial gene-mutation tests (Ames test) and chromosomal aberration assays in cultured cells. Emodin has also caused kidney tumours in male rats in long-term animal studies. These findings applied to the unrefined plant extract, not to food-grade cassia gum that meets the specification, but they drove the decision to require tight purity controls before EU and UK authorisation.
Emodin, a cassia-derived anthraquinone, tested positive for genotoxicity in in vitro assays and caused renal tubular tumours in male rats in a 2-year National Toxicology Program study.
2001AnimalNational Toxicology Program, Technical Report on Emodin
Multiple hydroxyanthraquinones present in cassia seed extracts showed mutagenic activity in Salmonella typhimurium Ames tests.
2006LabEFSA Panel on Food Additives, Scientific Opinion on cassia gum
Digestive effects at high intake
As a galactomannan dietary fibre, cassia gum is not absorbed and passes through the gut largely intact. At the small amounts present in food from permitted uses, it contributes negligible fibre. Very large doses of galactomannans can loosen stools, but this is a class effect of insoluble fibre at high intake and is not specific to the anthraquinone concern above.
Galactomannans including cassia gum are classified as dietary fibre; at food-additive use levels (up to 2.5g per kg of finished food) the dietary fibre contribution per typical serving is small and laxative effects are not expected.
2006Regulatory reviewEFSA Panel on Food Additives, Scientific Opinion on cassia gum