E-numbers / E163c Colour

Malvidin

also: Anthocyanidin (malvidin)
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The short version

A natural purple-red pigment from red grapes and blueberries, used to colour food and drinks. One of several anthocyanin compounds grouped together as E163.

What is it?

Malvidin is an anthocyanidin, a plant pigment in the flavonoid family. It is the compound primarily responsible for the red and purple colour of red wine grapes and is also abundant in blueberries and bilberries. In its natural form in fruit it exists as glycosides (malvidin bound to sugar molecules). As a food additive it is extracted from grape skins or other plant material and falls under the broader E163 anthocyanins approval. It is one of six anthocyanidin subtypes assigned letters (a through f) in reference literature, though in UK and EU food law all anthocyanins are authorised together under the single code E163 without separate sub-category approvals.

What does it do?

Malvidin absorbs light in the green-yellow range, producing visible red, purple, or blue hues depending on the acidity of the food. In acidic products it shows red-purple; in more neutral conditions it shifts towards blue. Like other anthocyanins, it is water-soluble and reasonably heat-stable in acidic environments, making it useful in coloured drinks, confectionery, and dairy products. It also acts as an antioxidant in the plant, scavenging free radicals, though how much of this activity persists after digestion in humans remains an open research question.

Where you will see it

Red grape skin extracts rich in malvidin are used in fruit-flavoured soft drinks, berry-flavoured yogurts, jams and fruit preparations, confectionery including sweets and jellies, ice cream, and some marbled cheeses. Blackcurrant extract (also listed as E163) contains different anthocyanins but is used in the same food categories. On a UK label, malvidin appears as part of the ingredient name 'anthocyanins', 'grape skin extract', or 'blackcurrant extract', often followed by the E number E163 in brackets.

What the science says

Regulatory assessment: no numerical ADI set because long-term data are thin

When EFSA re-evaluated E163 anthocyanins in 2013, its experts concluded the available toxicological database was not adequate to establish a numerical acceptable daily intake. Long-term studies on chronic toxicity, carcinogenicity, and reproductive effects were described as extremely limited. The panel noted that at current food-use levels, exposure from grape skin and blackcurrant extracts is not expected to pose a concern, but called for better characterisation and toxicological data to support a future re-evaluation. No ADI has since been set.

EFSA concluded the toxicological database for anthocyanins (E163) was inadequate to establish a numerical ADI; long-term chronic toxicity, carcinogenicity, and reproductive studies were extremely limited.

EFSA Panel on Food Additives and Nutrient Sources (ANS), EFSA Journal 2013;11(4):31452013regulatory review

Exposure from current use levels of aqueous grape skin extract and blackcurrant extract was not considered of safety concern, provided additive exposure was comparable to dietary intake of anthocyanins from fruit and vegetables.

EFSA Panel on Food Additives and Nutrient Sources (ANS), EFSA Journal 2013;11(4):31452013regulatory review

Antioxidant and anti-inflammatory activity in laboratory studies

Cell and test-tube studies show that malvidin glycosides reduce markers of inflammation in human endothelial cells and lower measures of oxidative stress. In one cell study, malvidin-3-glucoside suppressed inflammatory adhesion proteins by up to around 90% at high concentrations. These are laboratory findings, and whether the amounts of malvidin that actually reach human tissues after eating are sufficient to produce similar effects in the body is not established.

Malvidin-3-glucoside and malvidin-3-galactoside inhibited TNF-alpha-induced inflammatory adhesion molecules (ICAM-1, VCAM-1, MCP-1) in human umbilical vein endothelial cells in a dose-dependent manner.

Huang et al., Molecules, PMC62718302014lab

Malvidin and its glycosides demonstrated antioxidant and anti-inflammatory effects across multiple cell and animal studies, primarily attributed to modulation of NF-kappaB signalling and free-radical scavenging.

Latorres et al., Nutrients 2023;15(13):3016 (MDPI), PMC identifier 374473422023lab + animal

Cardiovascular signals from blueberry trials, but evidence is moderate quality

Randomised trials of whole blueberries, which are among the richest food sources of malvidin, show modest improvements in measures of blood vessel function. A 2024 meta-analysis of 11 trials found that blueberry intake improved flow-mediated dilation by around 1.5 percentage points, with moderate-quality evidence. The effect is attributed to the mixed anthocyanin content of blueberries rather than malvidin alone, and results show high variability between studies.

A meta-analysis of 11 randomised trials (400 participants) found blueberry intake improved flow-mediated dilation by 1.50% (95% CI: 0.81, 2.20; I-squared = 87%), rated as moderate-quality evidence.

Frontiers in Physiology, meta-analysis, doi: 10.3389/fphys.2024.13688922024meta-analysis

Potential anti-cancer signals in lab and animal studies, no human trial evidence

Laboratory and animal studies show malvidin can reduce cell proliferation and promote cell death in several cancer cell lines, including leukemia, colon, gastric, liver, and breast models. Researchers have not yet run controlled human trials to test whether malvidin has any effect on cancer risk or progression in people. These findings describe what happens in cultured cells or rodents, not what happens in someone who eats malvidin-containing food.

In vitro and animal studies show malvidin reduces cell proliferation, promotes apoptosis, and suppresses metastasis in cancer cell lines including leukemia, colorectal, gastric, liver, lung, and breast models.

Roriz et al., Journal of Functional Foods 2024 (ScienceDirect S1756464624001932)2024lab + animal

Authors noted that human clinical trials evaluating anticancer effects of malvidin are absent and called the compound's clinical translation at an early stage.

Roriz et al., Journal of Functional Foods 2024 (ScienceDirect S1756464624001932)2024lab + animal

Low bioavailability: most reaches the colon, not the bloodstream

Human studies show anthocyanins including malvidin are poorly absorbed. After consuming red wine containing malvidin-3-glucoside, plasma concentrations peaked at around 1 to 2 nanomolar within 20 minutes, which is very low. Most of what is eaten passes to the large intestine where gut bacteria convert it into smaller phenolic acids such as syringic acid, which are then absorbed. Whether these breakdown products contribute to any health effects is an active research question.

After ingesting red wine providing 68mg malvidin-3-glucoside, plasma concentration peaked at approximately 1.38 nM at 20 minutes, indicating very low bioavailability.

Bub et al., European Journal of Nutrition 2001, PMID 116974432001observational

Malvidin-3-glucoside is rapidly metabolised by colonic bacteria primarily to syringic acid, which is subsequently converted to gallic acid and protocatechuic acid; absorbed intact anthocyanin concentrations remain very low.

Gonzalez-Barrio et al., Journal of Agricultural and Food Chemistry 2012, doi: 10.1021/jf30021532012lab

Where it stands with the regulators

Status
Approved for use in the UK and EU as part of E163 (Anthocyanins)
Legal basis
UK FSA approved additives list (Great Britain, authorised as of 31 December 2020); assimilated Regulation (EC) No. 1333/2008 Annex II; specifications in assimilated Regulation (EU) No. 231/2012. Malvidin is not listed separately but is included within E163 anthocyanins. The EU and UK systems do not regulate individual anthocyanidin subtypes under distinct E numbers.
Permitted foods
Confectionery and sweets; Beverages including soft drinks and fruit drinks; Dairy products including yogurt and ice cream; Jams, marmalades, and fruit preparations; Glacé cherries; Soups; Pickles and condiments; Some marbled cheeses (red-marbled cheese)
Maximum levels
Quantum satis (no fixed numerical maximum; used at the level needed to achieve the intended colour, following good manufacturing practice) for most permitted categories. A specific maximum of 200mg/kg applies in fruit-flavoured breakfast cereals.
Safe-intake limit (ADI)
No numerical ADI set. EFSA (2013) concluded the toxicological database was insufficient to establish one. JECFA established an ADI of 0 to 2.5mg/kg body weight for grape skin extract specifically at its 26th meeting (1982), but this predated and is distinct from the EFSA 2013 review of E163 anthocyanins as a group.
History
Anthocyanins have been permitted in EU food since the original framework under EU Directive 94/36/EC (colours in foodstuffs). The 1975 Scientific Committee on Food opinion considered natural pigments from edible sources acceptable when dietary exposure was comparable to normal food intake. EFSA re-evaluated the whole E163 group in 2013 and called for additional long-term toxicological data. No bans or restrictions specific to malvidin have been issued. The additive is not one of the Southampton Six hyperactivity colours.

Who should be careful

No specific group has been identified as needing to avoid E163 anthocyanins in food. Malvidin is not a declarable allergen and is not associated with sulphite sensitivity, the Southampton Six, or any hormone-disrupting classification. People with known grape or berry fruit sensitivities may wish to check whether 'anthocyanins' or 'grape skin extract' appears in the ingredients list.

The honest read

Cutting through the noise

Malvidin is one of the most thoroughly researched individual anthocyanins, largely because it dominates red wine and is relatively easy to isolate. The laboratory evidence for anti-inflammatory and antioxidant effects is consistent, though it comes almost entirely from cell and animal studies. Human trials of whole blueberries (a rich malvidin source) show modest, real improvements in blood vessel measures, but the studies are heterogeneous and the benefits are attributed to a mixture of compounds, not malvidin alone. The biggest open question is bioavailability: so little malvidin reaches the bloodstream intact that researchers are still working out whether gut-metabolised breakdown products, rather than malvidin itself, drive any effects. EFSA's decision not to set a numerical ADI reflects genuine data gaps in long-term toxicology, not a discovered hazard. The research picture here is genuinely incomplete.

Related additives

Common questions

Is E163c banned in the UK?

No. E163c malvidin is permitted in the UK as part of the broader E163 (anthocyanins) approval. E163 is listed as a Group II colour, authorised at quantum satis, under the UK's assimilated version of EU Regulation 1333/2008. No ban or restriction applies specifically to malvidin.

Why did EFSA not set an acceptable daily intake for E163 anthocyanins?

In its 2013 re-evaluation, EFSA's Panel on Food Additives concluded that the toxicological database for E163 anthocyanins was too thin to support a numerical ADI. Long-term chronic toxicity and carcinogenicity studies were extremely limited. The panel noted that at current food-use levels, exposure was not expected to be of concern, but called for more data. No numerical ADI has been set since.

What foods contain E163c?

Malvidin is naturally present in red wine grapes, blueberries, bilberries, and elderberries. As an additive it reaches food via grape skin extract or anthocyanin extracts used to colour soft drinks, fruit-flavoured yogurts, jams, sweets and jellies, ice cream, and some confectionery. On the label it will appear as 'anthocyanins', 'grape skin extract', or sometimes 'E163'.

Is E163c vegan?

Yes. Malvidin and E163 anthocyanins are extracted from plant sources such as grape skins, blackcurrants, purple corn, and red cabbage. No animal-derived ingredients or processing aids are involved in the standard production routes.

Sources

Last reviewed: 20 June 2026

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