Too little safety data to set a limit
When European reviewers looked at E162 in 2015, they found the toxicity studies too limited to set a daily limit. No proper DNA-damage tests had been submitted, and there were no adequate long-term, cancer or reproduction studies. They still concluded it was not a concern at current use, mainly because betanin is already in the diet from eating beetroot and the additive supplies similar amounts.
The toxicity data were not enough to set a daily limit. Whether it damages DNA could not be judged, and there were no adequate long-term, cancer or reproduction studies.
2015Regulator reviewEFSA ANS Panel, Scientific Opinion on the re-evaluation of beetroot red (E 162) as a food additive, EFSA Journal 2015, volume 13, 4318
At reported use levels, reviewers did not see beetroot red as a concern, because its pigments are a normal part of the diet with a long history, and intake from the additive is similar to intake from food.
2015Regulator reviewEFSA ANS Panel, Scientific Opinion on the re-evaluation of beetroot red (E 162) as a food additive, EFSA Journal 2015, volume 13, 4318
Pink urine after eating it
About 10 to 14% of people pass pink or red urine after eating beetroot or food coloured with E162, because some of the pigment passes through undigested. It is more common in people short of iron, and it stopped in iron-deficient patients once they were treated. On its own it tells you the pigment passed through, nothing more.
Pink or red urine after eating beetroot affects about 10 to 14% of people, and is more common in people with iron-deficiency anaemia or poor absorption.
1993ObservationalWatson WC et al., Beeturia and the biological fate of beetroot pigments, Pharmacogenetics 1993, volume 3, 302-311
In seven iron-deficient patients, pink urine stopped after 8 days of iron treatment, pointing to how iron status affects the pigment's breakdown.
1993ObservationalWatson WC et al., Beeturia and the biological fate of beetroot pigments, Pharmacogenetics 1993, volume 3, 302-311
Most of it is not absorbed
In a human study, even after large amounts of beetroot juice or whole beetroot, betanin could not be found in the blood at any point. It seems to be broken down in the gut rather than absorbed whole.
Betanin was undetectable in blood after 250ml of beetroot juice (about 194mg betanin) or 300g of whole beetroot (about 66mg betanin).
2017Randomised trial in peopleClifford T et al., The plasma bioavailability of nitrate and betanin from Beta vulgaris rubra in humans, European Journal of Nutrition, PMC5346430
Antioxidant effects in the lab and in animals
In lab and animal studies, betanin acts as an antioxidant and dampens inflammation. Because so little reaches the blood in people, it is not established whether this happens at food colour amounts, and good human trials are lacking.
Betanin mopped up free radicals in lab tests and lowered signs of oxidative stress in rats.
2019Animal studyAllegra M et al., Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats, Antioxidants 2019, volume 8, 306, PMC6769636
Most evidence for betanin's antioxidant and anti-inflammatory effects comes from lab and animal work, and solid human trials are lacking.
2021ObservationalChen L et al., Beetroot as a functional food with huge health benefits, Food Science and Nutrition 2021, volume 9, 6158-6166